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کشاورزی نوین و پایدار - اندازه‎گیری پتانسیل اسمزی برگ به روش مارتینز (2004)
 
کشاورزی نوین و پایدار

 1- برگ رفرنس جدا شده از گیاه 24 ساعت در آب مقطر در سرد خانه و در دمای 4 درجه سانتیگراد قرار داده شد (برگ نباید تا خورده و سکسته شده باشد).

2- برگ را در هاون چینی قرار داده و سپس مقداری نیتروژن مایع روی آن اضافه کرده و با استفاده از هاون کاملاً خرد شد.

3- برگ خرد شده را در سیلندر سرنگ 2 میلی‎گرم که در انتهای سیلندر آن کاغذ صافی قرار داده شده بود ریخته و با قرار دادن پیستون سرنگ با فشار از آن عصاره گرفته و در ویال 1 میلی لیتری ریخته شد.

4-عصاره با دستگاه اسمومتر قرائت شده و با استفاده از فرمول زیر (معادله ونت هوف) عدد حاصله بهMpa  (مگاپاسکال) تبدیل گردید.

-Ψs=-MRIT

Ψs =پتانسیل اسمزی

M   =مولاریته، عدد حاصل از اسمو متر تقسیم بر 1000

R  = ثابت گازها برابر 0083143/0

I  =  ضریب یونیزاسیون  برابر 1

T  = دما بر حسب کالوین (۲۷۳ +۲۵  درجه سانتی گراد)

منبع:

فرانک طهماسبی. پیمان حسیبی.  موسی مسکرباشی. ۱۳۸۹. بررسی فیزیولوژیکی اثر آبیاری با آب شور از منابع NaCl و CaCl2 بر سه ژنوتیپ کلزا (Brassica napus L.)در شرایط آب و هوایی اهواز. پایان نامه کارشناسی ارشد. دانشگاه شهید چمران اهواز. ۱۱۴ صفحه.

Martinez, J. P., Lutls, S., Schanck, A., and Bajji, M. 2004. Is osmotic adjustment required for water stress resistance in the Mediterranean shrub Atriplex halmius L.? Plant Physiology, 161:1041-1051.





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